DETECTION OF HLA-B27 BY FLOW CYTOMETRY: RETROSPECTIVE STUDY IN A TERTIARY CARE HOSPITAL

Dr. Suhani Kunria, Dr. Anita Tahlan, Dr. Nayan Koul, Dr. S. Lehl

Abstract


Introduction: HLA-B27 is a class I major histocompatibility complex (MHC) antigen which is encoded on MHC region of chromosome 6 and a
non-MHC encoded beta chain, β2 microglobulin. It is associated with ankylosing spondylitis (AS) in 90-95% cases.
Material and Methods: All peripheral blood samples of spondyloarthropathy patients received for HLA-B27 typing were included in the study.
Cells were stained with monoclonal antibodies against HLA-B27 and CD3 using lyse wash procedure. Cell acquisition and analysis was done on
BD FACS Calibur flow cytometer.
Results: A total of 133 cases of spondyloarthropathy were studied over a period from 2008-2018 A total of 133 cases were studied . under this
period. These included 98 males (73.1%) and 36 females (26.8%) with Out of 133 cases, only 34 (25.5%) were reported as positive, 21 cases were
indeterminate (15.7%) and 78 cases were negative(58.6%).The flow cytometric analysis was repeated after 4 weeks in 21 indeterminate cases and
only 1 case(4.7%) came out to be positive.


Keywords


HLA-B27, Cytometry, Spondyloarthropathy, Major Histocompatibility Complex (MHC).

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